CyQuant Cell Proliferation Assay Kit, Apoptosis vs Necrosis and

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

CyQuant Cell Proliferation Assay Kit, Apoptosis vs Necrosis and Adherent cells were lysed and quantified by CyQuant® GR Dye as described in the Assay Protocol 1 Hynes, R O Cell 69, 11-25 References 2

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

cyquant protocol CyQuant assay protocol that we used In order to prepare the dilution that he used he starts by utilizing 400 fold CyQuant GR dye along with 20 fold

quantified by CyQuant® GR Dye as described in Assay Protocol References: Page 8 7 1 Ridley AJ, Schwartz MA, Burridge K, Firtel RA

cyquant protocol The assay employs CyQUANT GR dye, which produces a large fluorescence enhancement upon binding to cellular nucleic acids that can be measured using standard

CyQuant The total cellular DNA was determined using the CyQUANT™ Cell Proliferation Assay Kit (CyQUANT; Molecular Probes, Invitrogen