DEAE and CM Bio-Gel® A Ion Exchange Gels Instruction Manual

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DEAE-Sephacel chromatography of SGl fraction DEAE-Sephacel column was washed with molL of Tris HCI buffer, pt-l , and eiuted with 1

After this neutralization, each preparation of JH, JUL1 and JUL3 was loaded into a DEAE Sephacel anion-exchange column (5× 25 cm, Pharmacia Biotech, Uppsala Workflow of human aorta proteoglycan extraction, isolation by diethylaminoethyl –Sephacel chromatography and analysis LC-MSMS

11 विनर The activities of calpastatin, μ-calpain and m-calpain can be rapidly assayed following the separation on DEAE-Sephacel chromatography by a 2 stage elution with After this neutralization, each preparation of JH, JUL1 and JUL3 was loaded into a DEAE Sephacel anion-exchange column (5× 25 cm, Pharmacia Biotech, Uppsala